Flavanone 3-Hydroxylase Activity in Cultured Cells of Roselle(Hibiscus sabdariffa L.)
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منابع مشابه
Genetic Control of Flavanone 3-Hydroxylase Activity and Flavonoid 3'-Hydroxylase Activity in Antirrhinum majus (Snapdragon)
Anthocyanin Biosynthesis, Antirrhinum majus, Flavonoids, Flavanone 3-Hydroxylase, Flavonoid 3'-Hydroxylase, Genetic Control In flower extracts of defined genotypes of Antirrhinum majus, two different hydroxylases were found catalysing the hydroxylation of naringenin and eriodictyol in the 3-position and of naringenin in the 3'-position. The 3-hydroxylase is a soluble enzyme and belongs accordin...
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A key step in the biosynthesis of flavonoids is the hydroxylation of (2s)-flavanones in the 3 position to form the respective dihydroflavonols. The reaction is catalyzed by F30H, a soluble fraction enzyme requiring 2-oxoglutarate, oxygen, Fez+, and ascorbate for full activity (Heller and Forkmann, 1988). By using genetic mutants that lack F 3 0 H activity, cDNA clones have been identified that ...
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Flavonoid pigments are known to accumulate in red grains and coleoptiles of wheat and are synthesized through the flavonoid biosynthetic pathway. Flavanone 3-hydroxylase (F3H) is a key enzyme at a diverging point of the flavonoid pathway leading to production of different pigments: phlobaphene, proanthocyanidin, and anthocyanin. We isolated three F3H genes from wheat and examined a relationship...
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Recent studies with fibroblasts have shown that peptidylhydroxyproline formation begins toward the end of the log phase of growth and is an expression of the activity of the enzyme, collagen proline hydroxylase, which increase in late log-phase cells. This communication reports a two- to fivefold increase in hydroxylase activity on incubating early log-phase cells with lactate. The activation e...
متن کاملAnalysis of Flavanone 3-Hydroxylase in Arabidopsis Seedlings' Coordinate Regulation with Chalcone Synthase and Chalcone lsomerase
A genomic clone encoding flavanone 3-hydroxylase (F3H) was isolated from Arabidopsis thaliana. The deduced amino acid sequence is 72 to 94% identical to all previously reported F3H proteins. Low-stringency DNA blot analysis indicated that F3H is encoded by a single gene in Arabidopsis. The F3H locus was mapped to the bottom of chromosome 3 and therefore does not correspond to any of the 13 flav...
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ژورنال
عنوان ژورنال: Plant tissue culture letters
سال: 1992
ISSN: 1884-7706,0289-5773
DOI: 10.5511/plantbiotechnology1984.9.119